一抗 EP-CAM 鼠单抗 100ul REBIO G1111 包邮

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抗体来源	鼠单抗	产品类型	一抗
反应种属	Mouse , Human ,	产品应用	WB IHC ICC FC
储藏条件	-20度	产品性状	液体
分子量	35 kDa	克隆号	3-E1-E2
产品批号		产品浓度
保质期	12个月	产品品牌	REBIO
细胞定位		阳性对照
抗体类型
其他名称 抗原介绍 Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell-cell adhesion in epithelia. EpCAM is also involved in cell signaling, migration, proliferation, and differentiation. Additionally, EpCAM has oncogenic potential via its capacity to upregulate c-myc, e-fabp, and cyclins A & E. Since EpCAM is expressed exclusively in epithelia and epithelial-derived neoplasms, EpCAM can be used as diagnostic marker for various cancers. It appears to play a role in tumorigenesis and metastasis of carcinomas, so it can also act as a potential prognostic marker and as a potential target for immunotherapeutic strategies. Mutations in EpCAM have also been associated with congenital tufting enteropathy which causes intractable diarrhea in newborn children. 保存缓冲液 1*TBS (pH7.4), 0.5%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. 特殊性 This antibody is produced by immunizing mice with recombinant protein corresponding to a region of EpCAM.

应用介绍
Positive control: Hela, NIH/3T3, MCF-7, A431, SW1990, human colon carcinoma tissue, human breast carcinoma tissue, human kidney tissue, mouse kidney tissue   Subcellular location: Cell membrane prefix = o Recommended Dilutions: WB: 1:1,000-1:2,000    ICC: 1:100-1:200 IHC: 1:200-1:500       FC: 1:100-1:200 Fig1: Western blot analysis of EpCAM on different cell lysates using anti- EpCAM antibody at 1/1000 dilution. Positive control:   Lane 1: Hela      Lane 2: NIH/3T3 Lane 3: MCF-7 Fig2: ICC staining EpCAM in D3 cells (red). Cells were fixed in paraformaldehyde,  permeabilised with 0.25% Triton X100/PBS. Fig3: ICC staining EpCAM in A431 cells (green). The nuclear counter stain is DAPI (blue).  Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. Fig4: ICC staining EpCAM in SW1990 cells (green). The nuclear counter stain is DAPI (blue).  Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. Fig5: Immunohistochemical analysis of paraffin-embedded human colon carcinoma  tissue using EpCAM antibody. Counter stained with hematoxylin. Fig6: Immunohistochemical analysis of paraffin-embedded human breast carcinoma  tissue using EpCAM antibody. Counter stained with hematoxylin. Purity: ProL affinity purified